1. C. trachomatis is able to survive by: a) avoiding the lysosomal pathway b) av
ID: 101084 • Letter: 1
Question
1. C. trachomatis is able to survive by:
a) avoiding the lysosomal pathway
b) avoiding the host cell
c) avoiding the golgi
d) avoiding intermediate filaments
2. What biological molecule is detected using tissues for Immunohistochemistry?
3. If you wanted to determine the molecular size of a protein, which of the following techniques should you use?
a) Microarray
b) Immunohistochemistry
c) Western blot
d) Northern blot
4. Which of the following is/are transgenic animals?
a) GFP expressing mice
b) K-Creb expressing mice
c) p-Creb expressing mice
d) All of the above
e) A and B
Explanation / Answer
1C. trachomatis is able to survive by: avoiding the lysosomal pathway.This bacteria remains inside the endosome.Hence it can avoid lysomal secretion of the host.
2.Immunohistochemistry (IHC) refers to the process of selectively imaging antigens (e.g. proteins) in cells of a tissue section by exploiting the principle of antibodies binding specifically to antigens in biological tissues.
3.c.The western blot (sometimes called the protein immunoblot) is a widely used analytical technique used in molecular biology, immunogenetics and other molecular biology disciplines to detect specific proteins in a sample of tissue homogenate or extract.
4.d.Transgenic mice were produced using the GFP coding sequence, c AMP response element protein is expressed in many animals including humans and is used to construct a genetically enginered mice,k CREB a mutant form of CREB though does not binds to DNA but dimerizes with other family members of CREB was also found to be expressed.p CREB the phosphorylated form of CREB was also used to make a trasgenic mice.
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