A specific mutation in the promoter of the gene that encodes the enzyme Isocitra

Question

A specific mutation in the promoter of the gene that encodes the enzyme Isocitrate dehydrogenase causes the

promoter to become non-functional, and unable to bind to RNA Polymerase. I take equal numbers of wild type

cells and cells that are heterozygous for the mutation, and measure the kinetics of Isocitrate dehydrogenase in

the cells. My most likely prediction for the observed Km and V max is:

A) Km and Vmax will be the same in both wild type and mutant cells

b)Km and Vmax will be higher in the wild type compared to mutant cells

c) Km will remain the same and Vmax will be higher in the wild type compared to mutant cells

d)Vmax will remain the same and Km will be higher in the mutant

Explanation / Answer

ANS: B. Km and Vmax will be higher in the wild type compared to mutant cells.

Explanation: Km is the concentration of substrate which permits the enzyme to achieve half Vmax. An enzyme with a high Km has a low affinity for its substrate, and requires a greater concentration of substrate to achieve Vmax.

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