You are trying to reproduce experimental data from the previous student in the l

Question

You are trying to reproduce experimental data from the previous student in the lab. He/she had reported that the enzyme under investigation has a k_cal of 1875 s^-1 and a catalytic efficiency of 7.5 10^7 M^-1 s^-1. What is the Km of this enzyme? In repeating the measurement, you want to have a vmax of 10 mM s^-1 (as this is easy to measure). How much enzyme should you use? Provide your result in muM. Calculate at which substrate concentrations in muM you should measure the initial rate v_0 to obtain the following values: Create a predicted Michaelis-Menten plot for your enzyme. Draw a predicted Lineweaver-Burke plot for your enzyme.

Explanation / Answer

a) catalytic efficiency(e)  is defined as kcat / KM

e =  kcat / KM

KM =  kcat / e

we know the two values

So, KM = 1875 / 7.5 *107 = 250 * 10-7 M = 25 * 10-6 M

vmax = kb * [E0]

we know that kcat = kb

Hence vmax = kcat * [E0]

[E0] =  vmax /  kcat = 10 / 1875 mM = 5.33 * 10-3 mM = 5.33 uM.

C) Now, you have everything required in Michaelis-Menten equation. Just sustitute the values in the Michaelis-Menten equation and find the values of [Substrate] for each value of v0 .

Thank You

Thank You

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