Question: average count of 42 cells/mm2 grid is counted in a hemacytometer for a

Question

Question: average count of 42 cells/mm2 grid is counted in a hemacytometer for a 2.5 ml suspension of cells. (Show your calculations!)

• What is the cell concentration of the suspension?

• Approximately how many cells are in the entire sample?

• To perform an experiment, 8 dishes of cells with 1.2 x 105 cells per dish are needed. How many ml of the suspension will be needed for the experiment? Are there enough cells?

• How many cells would have to be actually added to a plate (this is usually called “seeded”) to begin a culture with 3.5 x 105 cells/dish, if the line of cells is known to have a plating efficiency of 65%? (Show your calculations!)

Explanation / Answer

1 ml contains 10 mm. So, 2.5 ml is equal to 25 mm.

1 sq.mm of the grid contains 42 cells.

So, 5 sq.mm (25 mm) contains 210 cells

1) 2.5 ml contains 210 cells. Therefore, the concentration of the suspension is,

= 210/2.5

= 84 cells/ml

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