You are interested in cloning the human insulin gene and so you create a genomic

Question

You are interested in cloning the human insulin gene and so you create a genomic library of human DNA from the DNA of human white blood cells. You isolate fragments of human genomic DNA and then ligate those fragments into vectors. The image below represents the vector that you've chosen for cloning. Once you've completed the ligation reaction, you transform E. coli cells (originally ampR^-, lacZ) with the population of vectors. If you plate the resulting cells on medium containing ampicillin, which of the following types of cells will grow? Circle all that apply. a. cells containing recombinant plasmid b. cells containing nonrecombinant plasmid c. untransformed cells If you also plate cells on the pigment compound that is turned blue by B-galactosidase, which of the following types of cells will appear blue? Circle all that apply. a. cells containing recombinant plasmid b. cells containing nonrecombinant plasmid c. untransformed cells

Explanation / Answer

1)-a cells containing recombinant plasmid and b cells containing nonrecombinant plasmid.

The e coli cells don't have AMPr site and plasmid contain AMPr site and the only cells that will which have the plasmid and those are recombinant cells.

The cells which have nonrecombinant plasmid will also grow because the cell still have the plasmid which have AMPr site.

2) b) cells containing recombinant plasmid and c) untransformed cells. Because recombinant plasmid is inserted in between the lacz site and it will. Make white colonies since it will not make beta galactosidase.

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