1) The specificity of an exoglycosidase (enzyme that cleaves the non-reducing re

Question

1) The specificity of an exoglycosidase (enzyme that cleaves the non-reducing residue of a

polysaccharide chain) was tested on several substrates. The structure of the substrate (a to i) is

shown below. Note that: G = N-acetyl glucosamine, M = mannose, and Gr = reduced Nacetylglucosamine.

One mole of N-actylglucosamine is released after treatment of a, b, g or h with this enzyme. Two

moles of N-actylglucosamine are released after treatment of molecules e and f. The enzyme does

not modify molecules c, d, and i.

Determine the specificity of the enzyme. Be as precise as possible. (6pts)

2) How many moles of N-actylglucosamine are released after treatment of molecules j and k? (2

pts)

Explanation / Answer

1.)The following can be inferred about the specificity of exoglucosidase:-

So, this enzyme is specific for Beta 1-2 bond between G and M. But cannot cleave this bond when the same mannose involved in B 1-2 bond is involved in B 1-6 bond also, with different M.

2.) For both J and K Only one mole of G will be released, when only beta 1-2 bond is broken, and no other bond.

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