1. You receive a mixture of two dyes in lab with directions to determine the qua
ID: 1004778 • Letter: 1
Question
1. You receive a mixture of two dyes in lab with directions to determine the quantity of each dye in the solution. You receive standard curves for all four dyes and the absorbance spectrum at the right which shows the maximum absorbance for all four dyes.
a. Based on this spectrum, which of the following dye mixtures would give you the most accurate data for the concentrations of the two dyes when analyzed in a mixture? Briefly explain why.
o Red at 520 nm and yellow at 430 nm
o Blue at 630 nm and green at 630 nm
o Red at 520 nm and green at 630 nm
Explanation / Answer
Red at 520 nm and green at 630 nm because the absorption maxima are the farthest apart reducing the possibility of any spectral interference.
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