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1. Select all that apply. An enzyme -is a carbohydrate -is a protein -is a biolo

ID: 1001683 • Letter: 1

Question

1. Select all that apply. An enzyme

-is a carbohydrate
-is a protein
-is a biological catalyst
-is changed by reaction with a substrate
-is not altered by reaction with a substrate
-can be reused if not damaged
-increases the rate of chemical reaction
-slows the rate of chemical reactions
-lowers the activation energy of a reaction
-increases the activation energy of a reaction

2. Select all that apply. An enzyme

-is made of protein
-increases the rate of chemical reaction
-can be reused if not damaged
-slows the rate of chemical reactions
-is made of carbohydrate
-is not altered by reaction with a substrate
-is changed by reaction with a substrate

Chrome File Edit View History Bookmarks People Window Help 45% 0, Sun 10:19 PM Brianna Jimenez a E A Experiment 3: a-Glucosida x Cchegg Study | Guided Solu x Brianna ? ? fi https://www.webassign.net/web/Student/Assignment-Responses/last?dep= 13779708 Submit Answer Save Progress 3. + -134.34 points My Notes Ask Your Teacher You run an enzyme assay designed to determine the ?-glucosidase activity after 1 hour (60 min) by determining the concentration of p-nitrophenol. The results you collected are presented below Part 1 Enzyme Dry Weight Absorbance at 580 nm 0.270 The Required dilution factor: 35 Part 2 p-nitrophenol absorbance at 400 nm: 0.731 You now need to analyze the results and calculate the following: (Answer each question to 3 significant figures) Part 1 Calculate the Dry Weight (DW) of your enzyme using the following equation DWenzyme = 0.35 . A580 . DF = 9 If the molar absorptivity of the p-nitrophenol is 7.28 mMcm and the path length is 1.0 cm, what is the concentration of p-nitrophenol in the sample? mM Using the formula below, what is the activity of your enzyme? Activity = 0.00728 * DWenzyme A400 * 1000 (Hmol/min.g) 60

Explanation / Answer

Ans 1. An enzyme :

1. Is a protein.

2. Is a biological catalyst .

3. Is not altered by reaction with a substrate .

4 .Can be reused if not damaged .

5 . Increases the rate of chemical reaction .

6. Lowers the activation energy of a chemical reaction .

Ans 2 . An enzyme :

1. Is made of protein .

2. Increases the rate of chemical reaction .

3. Can be reused if not damaged .

4. Is not altered by reaction with a substrate .