Using a capillary electrophoresis apparatus consisting of a 30cm fused silica ca

Question

Using a capillary electrophoresis apparatus consisting of a 30cm fused silica capillary and an applied voltage of 10kV with a buffer of pH=9 you identify a compound of interest as having a migration time of t_R=10min. The injection happens at the anode (the positive electrode) and the detector is close to the cathode (the negative electrode). In this system a small molecule not carrying a charge has a migration time of t_M=5 minutes. Can you deduce whether your molecule carries a positive or negative charge? Since the molecule takes longer to show up, it must be negatively charged Since the molecule takes longer to show up, it must be positively charged You can't tell the charge of the molecule from this experiment

Explanation / Answer

Solution :-

Cathode is the negative electrode and detector is near to cathode therefore since the time taken by themolecule is 5 minutes

so this means the molecule takes longer to show up means it is negatively charged.

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